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pe streptavidin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pe streptavidin
    Pe Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptavidin+pe/Streptavidin%2C+Streptomyces+avidinii/pmc13136873-87-11-13
    Average 99 stars, based on 1 article reviews
    pe streptavidin - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Fusobacterium nucleatum Lipopolysaccharides Disrupt the Interaction of Siglec‑7 to Sialoglycans Expressed on Mammalian Cells
    Article Snippet: .. The expression of sialic acids was monitored by flow cytometry following the addition of biotinylated Maackia amurensis lectin II (MALII) or Sambucus nigra lectin-I (SNA-I) (Invitrogen) (0.04 μg/mL) to the untreated cells or to cells pretreated with sialyltransferase inhibitor Ac 5 -SiaNEtocF at concentrations of 10 μM, and surface sialylation levels were then evaluated using biotinylated lectin (SiaFind Pan-Specific Lectenz Kit, Lectenz Bio) (0.04 μg/mL) followed by streptavidin-PE (2 μg/mL) (Invitrogen, eBioscience). .. For each condition, cell suspensions were analyzed by flow cytometry, and fluorescence was recorded using the CytoFlex flow cytometer plate-reader function (Beckman Coulter, CA).

    Flow Cytometry:

    Article Title: Fusobacterium nucleatum Lipopolysaccharides Disrupt the Interaction of Siglec‑7 to Sialoglycans Expressed on Mammalian Cells
    Article Snippet: .. The expression of sialic acids was monitored by flow cytometry following the addition of biotinylated Maackia amurensis lectin II (MALII) or Sambucus nigra lectin-I (SNA-I) (Invitrogen) (0.04 μg/mL) to the untreated cells or to cells pretreated with sialyltransferase inhibitor Ac 5 -SiaNEtocF at concentrations of 10 μM, and surface sialylation levels were then evaluated using biotinylated lectin (SiaFind Pan-Specific Lectenz Kit, Lectenz Bio) (0.04 μg/mL) followed by streptavidin-PE (2 μg/mL) (Invitrogen, eBioscience). .. For each condition, cell suspensions were analyzed by flow cytometry, and fluorescence was recorded using the CytoFlex flow cytometer plate-reader function (Beckman Coulter, CA).

    Article Title: CD47/pd-L1-targeting protein complex and methods of use thereof
    Article Snippet: .. After washing, streptavidin-PE (eBioscience, Cat #: EBS12-4317-87) was added at 0.3 μg per well, and PE signals from the cells were analyzed using a CytoFLEX flow cytometer (Beckman Coulter Inc.). ..

    Labeling:

    Article Title: Biophysical trade-offs in antibody evolution are resolved by conformation-mediated epistasis
    Article Snippet: The total volume of the labeling reaction was adjusted to limit ligand depletion effects to <10%, ranging from 50 μL for 100 nM RBD to 1 L for 10 pM RBD. .. Following labeling with RBD, cells were pelleted by centrifugation at 4°C (for large volumes, cells were spun at 300g for 10 min; smaller volumes were spun for 5 min), washed twice with FACS buffer and stained with streptavidin-PE (1:50; ThermoFisher S866) and myc-FITC (1:50; Miltenyi Biotec 130-116-485) by incubating for 1 hour at 4°C in the dark with gentle rocking. ..

    Centrifugation:

    Article Title: Biophysical trade-offs in antibody evolution are resolved by conformation-mediated epistasis
    Article Snippet: The total volume of the labeling reaction was adjusted to limit ligand depletion effects to <10%, ranging from 50 μL for 100 nM RBD to 1 L for 10 pM RBD. .. Following labeling with RBD, cells were pelleted by centrifugation at 4°C (for large volumes, cells were spun at 300g for 10 min; smaller volumes were spun for 5 min), washed twice with FACS buffer and stained with streptavidin-PE (1:50; ThermoFisher S866) and myc-FITC (1:50; Miltenyi Biotec 130-116-485) by incubating for 1 hour at 4°C in the dark with gentle rocking. ..

    FACS:

    Article Title: Biophysical trade-offs in antibody evolution are resolved by conformation-mediated epistasis
    Article Snippet: The total volume of the labeling reaction was adjusted to limit ligand depletion effects to <10%, ranging from 50 μL for 100 nM RBD to 1 L for 10 pM RBD. .. Following labeling with RBD, cells were pelleted by centrifugation at 4°C (for large volumes, cells were spun at 300g for 10 min; smaller volumes were spun for 5 min), washed twice with FACS buffer and stained with streptavidin-PE (1:50; ThermoFisher S866) and myc-FITC (1:50; Miltenyi Biotec 130-116-485) by incubating for 1 hour at 4°C in the dark with gentle rocking. ..

    Staining:

    Article Title: Biophysical trade-offs in antibody evolution are resolved by conformation-mediated epistasis
    Article Snippet: The total volume of the labeling reaction was adjusted to limit ligand depletion effects to <10%, ranging from 50 μL for 100 nM RBD to 1 L for 10 pM RBD. .. Following labeling with RBD, cells were pelleted by centrifugation at 4°C (for large volumes, cells were spun at 300g for 10 min; smaller volumes were spun for 5 min), washed twice with FACS buffer and stained with streptavidin-PE (1:50; ThermoFisher S866) and myc-FITC (1:50; Miltenyi Biotec 130-116-485) by incubating for 1 hour at 4°C in the dark with gentle rocking. ..

    Article Title: Specific targeting of MR1-antigen complexes using nanobodies
    Article Snippet: .. After three washes, yeast were then stained with cocktail containing Streptavidin-PE (Molecular Probes) at 1 mg ml -1 , anti-HA AF488 (1:200) and Live-Dead Near Infrared (1:500) for 15 minutes at RT. ..

    Gentle:

    Article Title: Biophysical trade-offs in antibody evolution are resolved by conformation-mediated epistasis
    Article Snippet: The total volume of the labeling reaction was adjusted to limit ligand depletion effects to <10%, ranging from 50 μL for 100 nM RBD to 1 L for 10 pM RBD. .. Following labeling with RBD, cells were pelleted by centrifugation at 4°C (for large volumes, cells were spun at 300g for 10 min; smaller volumes were spun for 5 min), washed twice with FACS buffer and stained with streptavidin-PE (1:50; ThermoFisher S866) and myc-FITC (1:50; Miltenyi Biotec 130-116-485) by incubating for 1 hour at 4°C in the dark with gentle rocking. ..

    other:

    Article Title: Ultra-long acting insulin-Fc fusion proteins and methods of use
    Article Snippet: The % inhibition by test compounds of biotinylated-RHI binding on IM-9 cells was plotted against log concentrations of the test compound, and the resulting IC50 values were calculated using GraphPad Prism (GraphPad Software, La Jolla, CA) for the test compounds.

    Article Title: Ultra-long acting insulin-Fc fusion proteins and methods of use
    Article Snippet: The % inhibition by test compounds of biotinylated-RHI binding on IM-9 cells is plotted against log concentrations of the test compound, and the resulting IC50 values are calculated using GraphPad Prism (GraphPad Software, La Jolla, CA) for the test compounds.

    Article Title: Mixed binding domains
    Article Snippet: moFc-biotin fusion, Biotin-labeled Bioscience (Mouse) HiP Cell Selections Cell selections are performed with the KingFisher essentially according to the manufacturer's instructions, with following remarks/changes: Selections may be done using FreeStyle 293-F cells that are transiently transfected with an expression vector encoding huPD-1 or moPD-1.

    Incubation:

    Article Title: Dynamic changes in serum HER2-peptide-specific autoantibodies predict response to neoadjuvant therapy in HER2-positive breast cancer.
    Article Snippet: The beads were washed and incubated with 100 μL of x200 diluted biotinconjugated goat anti-human IgG (BA-3080; Vector Laboratories, Burlingame, CA) for 1 hour at room temperature on a plate shaker. .. After washing, 100 μL of 5 μg/ml streptavidin-PE (Invitrogen, Carlsbad, CA) was added to the wells, and the plate was incubated for 30 minutes at room temperature on a plate shaker. ..



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